WEIGHT: 52 kg
Sex services: Golden shower (in), Striptease amateur, Massage, Hand Relief, Smoking (Fetish)
Neural induction was carried out with a small molecule cocktail based two-step culture protocol, over a total duration of 14 days. At the 8 and 14 day timepoints, the cells were analyzed for expression of neural markers with immunocytochemistry, qRT-PCR and Western Blot. The Fluo 4-AM calcium flux assay was also performed after a further 14 days of neural maturation.
More pronounced morphological changes characteristic of the neural lineage i. This was corroborated by the immunocytochemistry, qRT-PCR and western blot data, which showed upregulated expression of several early and mature neural markers in all three cell types treated with small molecules, versus the corresponding untreated controls.
Read Article at publisher's site. How does Europe PMC derive its citations network? CitePeer Related Articles http: Gene Ontology GO Terms.